Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis

Parasites & Vectors

Table 1 Anti-Trichinella immunoglobulin G levels in serum samples collected from mice infected with T. spiralis and other parasites, by the recombinant T. spiralis trypsin-enyzme-linked immunosorbent assay (ELISA) and the excretory/secretory antigens-ELISA

Worm species^a	No. of serum samples^b	rTsTryp-ELISA		ES antigens-ELISA
Worm species^a	No. of serum samples^b	OD value (mean ± SD)	No. of positive samples (%)	OD value (mean ± SD)	No. of positive samples (%)
Trichinella spiralis	20	2.01 ± 0.43	20 (100)	2.37 ± 0.22	20 (100)
Angiostrongylus cantonensis	12	0.10 ± 0.03	0 (0)	0.07 ± 0.04	0 (0)
Capillaria hepatica	10	0.09 ± 0.04	0 (0)	0.08 ± 0.03	0 (0)
Clonorchis sinensis	20	0.12 ± 0.04	0 (0)	0.10 ± 0.05	0 (0)
Schistosoma japonicum	24	0.14 ± 0.05	0 (0)	0.14 ± 0.05	0 (0)
Spirometra mansoni	30	0.09 ± 0.04	0 (0)	0.10 ± 0.05	1 (3.3)
Toxoplasma gondii	15	0.04 ± 0.02	0 (0)	0.04 ± 0.02	0 (0)
Uninfected mice	50	0.11 ± 0.02	0 (0)	0.12 ± 0.04	0 (0)

ES Excretory/secretory, OD optical density, rTsTryp recombinant T. spiralis trypsin, SD standard deviation
^aThe parasite infecting dose per mouse was: 200 T. spiralis ML, 30 A. cantonensis third-stage larvae, 200 C. hepatica infective eggs, 60 C. sinensis metacercaria, 100 S. japonicum cercariae, 5 S. mansoni plerocercoids, and 4 × 10⁴ T. gondii (RH strain) tachyzoites
^bSerum samples of mice infected with T. spiralis, A. cantonensis, C. sinensis, S. japonicum, S. mansoni and T. gondii were collected at 30, 21, 84, 42, 42, 30, and 30 days post-infection, respectively

ISSN: 1756-3305