Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis

Parasites & Vectors

Table 4 Anti-Trichinella immunoglobulin G levels in serum from trichinellosis patients by the recombinant T. spiralis trypsin-enyzme-linked immunosorbent assay (ELISA) and the excretory/secretory antigens-ELISA

Sera of patients with	No. of serum samples	rTsTryp-ELISA		ES antigens-ELISA
Sera of patients with	No. of serum samples	OD value (mean ± SD)	No. of positive samples (%)	OD value (mean ± SD)	No. of positive samples (%)
Trichinellosis	52	0.91 ± 0.37	51 (98.1)	0.97 ± 0.34	49 (94.2)
Early trichinellosis^a	20	0.95 ± 0.38	19 (95)	0.92 ± 0.48	17 (85)
Late trichinellosis^b	32	0.89 ± 0.36	32 (100)	1.00 ± 0.21	32 (100)
Hepatic capillariasis	5	0.06 ± 0.02	0	0.08 ± 0.02	0
Filariasis	12	0.24 ± 0.06	0	0.26 ± 0.07	0
Ancylostomiasis	3	0.08 ± 0.05	0	0.11 ± 0.05	0
Paragonimiasis	25	0.15 ± 0.06	0	0.16 ± 0.09	1 (4)
Schistosomiasis	30	0.19 ± 0.07	0	0.26 ± 0.13	2 (6.7)
Clonorchiasis	11	0.21 ± 0.11	1 (9.1)	0.23 ± 0.12	1 (9.1)
Cysticercosis	20	0.21 ± 0.08	1 (5)	0.31 ± 0.16	3 (15)
Echinococcosis	24	0.20 ± 0.11	1 (4.2)	0.28 ± 0.14	2 (8.3)
Sparganosis	8	0.19 ± 0.07	0	0.26 ± 0.19	2 (25)
Healthy individuals	100	0.17 ± 0.08	0	0.20 ± 0.09	0

ELISA Enzyme-linked immunosorbent assay, ES excretory/secretory, OD optical density, rTsTryp recombinant T. spiralis trypsin, SD standard deviation
^aEarly trichinellosis: serum samples of trichinellosis patients were obtained at 19 days post-infection
^bLater trichinellosis: serum samples of trichinellosis patients were obtained at 35 days post-infection

ISSN: 1756-3305