Table 1 Overview of quantitative real-time polymerase chain reaction (qPCR) Toxoplasma gondii-positive squirrels (n = 178) per sampling year, season, and region, with odds ratios (OR) from univariable logistic regression analysis

Sampling year

 2014

52.9 (18/34; 35.3–70.2)

1

–

 2015

33.3 (9/27; 16.5–54.0)

0.44 (0.15–1.24)

0.128

 2016

30.0 (6/20; 11.9–54.3)

0.38 (0.11–1.19)

0.106

 2017

20.0 (3/15; 4.3–48.1)

0.22 (0.04–0.85)

0.040*

 2018

15.4 (2/13; 1.9–45.4)

0.16 (0.02–0.72)

0.030*

 2019

22.2 (8/36; 10.1–39.1)

0.25 (0.09–0.70)

0.009*

 2020

9.1 (3/33; 1.9–24.3)

0.09 (0.02–0.31)

 < 0.001*

Season

 Autumn (September–November)

44.4 (32/72; 32.7–56.6)

1

–

 Winter (December–February)

8.0 (2/25; 0.98–26.0)

0.11 (0.02–0.40)

0.004*

 Spring (March–May)

27.1 (13/48; 15.3–41.8)

0.46 (0.21–1.01)

0.056

 Summer (June–August)

6.1 (2/33; 0.74–20.2)

0.08 (0.01–0.29)

0.001*

Region

 East

29.9 (23/77; 20.0–41.3)

1

–

 South

28.2 (11/39; 15.0–44.9)

0.92 (0.38–2.13)

0.852

 West

16.7 (5/30; 5.6–34.7)

0.47 (0.14–1.30)

0.169

 North

31.2 (10/32; 16.1–50.0)

1.07 (0.42–2.57)

0.886