Fig. 2
Relative abundances of Leishmania species identified in the samples analyzed. A, B Relative abundance of Leishmania species identified in the samples analyzed using HSP70-Long MinION™ sequencing (A) and HSP70-Short MinION™ sequencing (B). Mix refers to the mixtures of L. infantum and L. amazonensis made in the laboratory: Mix-1 contained 1 × 106 parasites/ml of L. infantum and 1 × 103 parasites/ml of L. amazonensis; Mix-2 contained 1 × 103 parasites/ml of L. infantum and 1 × 106 parasites/ml of L. amazonensis. Color bars represent the Leishmania species found in the sample, HSP70-Long, 771-bp 70-kDa heat shock protein gene-based marker; HSP70-Short, previously published heat shock protein gene-based marker spanning 330 bp.