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Fig. 1 | Parasites & Vectors

Fig. 1

From: Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix

Fig. 1

Microscopy observation and SDS-PAGE analysis of the purified WFBs and the oocyst wall. a Immunofluorescence co-staining of the WFB-rich extract incubated with rabbit anti-rEnGAM22 pAb (visualized with FITC, green) and mouse anti-rEnGAM59 pAb (visualized with Cy3, red), showing that the enriched fractions include both intact WFB1s and intact WFB2s. b Purified oocyst wall of Eimeria necatrix observed by light microscope. c SDS-PAGE analysis of three biological repetitions of WFBs and OWs. Lanes: M, Marker; WFBs-1/WFBs-2/WFBs-3, WFB replicates 1/2/3; OW-1/OW-2/OW-3, OW replicates 1/2/3. Scale bars: 10 μm. Anti-rEnGAM22/-rENGAM59, Anti-recombinant E. necatrix GAM protein 22/GAM protein 59; GAM, Gametocyte(s); OW, oocyst wall; pAb, polyclonal antibody; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; WFB1/2, wall-forming body type 1/type 2

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Parasites & Vectors

ISSN: 1756-3305

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