Fig. 3

Overview of tandem mass tag-based quantitative proteomics for quantification. a Volcano plot of quantified proteins in the proteome database for WFBs and OW. The plot depicts log2 (fold change) on the horizontal axis, with a dashed line indicating the fold-change cutoff. The vertical axis represents log10 (P-value), and the dashed line represents the P-value cutoff. Red dots represent upregulated proteins in the OW group (fold change ≥ 1.5, P < 0.05); green dots represent downregulated proteins in the oocyst wall group (fold change ≤ 0.667, P < 0.05); black dots represent proteins with no significant difference in expression (0.667 < fold change < 1.5 or P > 0.05). B Clustering analysis of the differentially expressed proteins in WFBs and oocyst wall. Lanes: 1, 2, 3, the three biological replicates of the OW; 4, 5, 6, the three biological replicates of WFBs. OW, Oocyst wall; WFBs, wall-forming bodies