Fig. 4

Validation analysis of EnGAM22 and EnGAM59 by immunoblotting (Simple Western analysis). A1, B1 Protein expression levels of EnGAM22 and EnGAM59 in the WFBs and OW groups were analyzed using the capillary-based Simple Western automated system (ProteinSimple) and presented as virtual blots. A2, B2 Relative peak areas of EnGAM22 and EnGAM59 in the WFBs and OW groups were determined using the capillary-based Simple Western automated system. EnGAM22/EnGAM59, E. necatrix GAM protein 22/GAM protein 59; OW, oocyst wall; WFBs, wall-forming bodies