Skip to main content
Fig. 8 | Parasites & Vectors

Fig. 8

From: Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix

Fig. 8

Expression and western blot analysis of rEnPDI, rEnTrx and rEnPGK. a1-a3 Western blot detection of the recombinant proteins. rEnPDI (A1, approx. 57 kDa), rEnTrx (A2, approx. 52 kDa) and rEnPGK (A3, approx. 45 kDa) could be specifically recognized by the anti-6×His tag monoclonal antibody with the specific bands. Lanes: M, Protein marker; 1, BL21(DE3) cells transformed with recombinant vector and induced by 1 mM IPTG; 2, BL21 (DE3) cells transformed with the empty vector and induced by 1 mM IPTG; 3, BL21(DE3) cells induced with 1 mM IPTG. b1-b3 Native proteins extracted from second-generation merozoites, third-generation merozoites, gametocytes, unsporulated oocysts and sporulated oocysts) were detected by mouse anti-rEnPDI (B1), rEnTrx (B2) and rEnPGK (B3) polyclonal antibody. Lanes: M, Protein marker; 1, second-generation merozoites; 2, third-generation merozoites; 3, gametocytes; 4, unsporulated oocysts; 5, sporulated oocysts. rEnPDI/rEnTrx/rEnPGK, Recombinant E. necatrix disulfide isomerase/thioredoxin/phosphoglycerate kinase

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us