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Fig. 9 | Parasites & Vectors

Fig. 9

From: Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix

Fig. 9

Immunofluorescence analysis of macrogametes in tissue sections (a, b, e, f, i, j) and in freshly harvested (c, d, g, h, k, l) of E. necatrix. Immunolabeled with mouse anti-rEnPDI (a-d), mouse anti-rEnTrx (e–h) and mouse anti-rEnPGK (i-l). Anti-rEnGAM22 rabbit sera (a, c, e, g, i, k) served as counterstaining of WFB1s, and anti-rEnGAM59 rabbit sera (b, d, f, h, j, l) served as counterstaining of WFB2s. Scale bar: 10 μm. Anti-rEnGAM22/-rENGAM59, Anti-recombinant E. necatrix GAM protein 22/GAM protein 59; rEnPDI/rEnTrx/rEnPGK, recombinant E. necatrix protein disulfide isomerase/thioredoxin/phosphoglycerate kinase; WFB1/2, wall-forming body type 1/type 2

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