Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix

Table 1 Primer sequence

Primer name	Primer sequence 5ʹto 3ʹ^c
EnPDI-F^a	ATGAAAAGACCTTTCTTGCTCGG
EnPDI-R^a	TCAGAGTTCCTCGCCCTTGTC
EnPDI-pET28aF^b	CGGGATCCGCAGCAGCAGCAGCAGAGAACAA
EnPDI-pET28aR^b	CCGCTCGAGTCAGAGTTCCTCGCCCTTGTCGG
EnTrx-F^a	ATGGCTTTGGGCGTTGGCTTAGGAA
EnTrx-R^a	CTAAAGCTCTTCTTTCTTTGTGT
EnTrx-pET28aF^b	CGGGATCCGCGCGGCCGCTGATCGACCCCAT
EnTrx-pET28aR^b	CGGAATTCCTAAAGCTCTTCTTTCTTTGTGT
EnPGK-F^a	ATGCGCGTGGACTTCAACGTGCC
EnPGK-R^a	TCACTTTGAAGACAAAGCTGCCACTC
EnPGK-pET28aF^b	CGGGATCCATGCGCGTGGACTTCAACGTGCC
EnPGK-pET28aR^b	ATAGTTTAGCGGCCGCTCACTTTGAAGACAAAGCTG

En Eimeria necatrix, F forward primer, PDI protein disulfide isomerase, pET28a bacterial vector, PGK phosphoglycerate kinase, R reverse primer, Trx thioredoxin
^aPrimers for complete gene
^bPrimers for prokaryotic expression
^cThe protective bases are shown in italics and the restriction sites are underlined

ISSN: 1756-3305