Fig. 7

Protocols evaluated in both phases for the recovery of Taenia saginata eggs from the exoskeleton, including the time required to perform each step. A Comparison of two protocols involving different washing solutions, including PBS (E.I.1) and 0.05% Tween 80 (E.I.2). Following washing of the fly’s exoskeleton in either solution by vortexing, the fly was left undisturbed for 15 min before being removed from the tube. The contents of the tube were then centrifuged at 2000 g for 2 min. B The protocol was repeated as previously described (E.I.2); however, three different passive sedimentation durations were assessed: 15 (E.II.1), 5 (E.II.2) and 1 (E.II.3) min. Protocol notation: E, exoskeleton; I–II, phase during which the protocol was evaluated; 1–3, protocol within that phase. PBS, Phosphate-buffered saline