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Table 1 Recovery rate and time to recover Taenia saginata eggs from the gastrointestinal tract of house flies for 13 protocols across four phases

From: Performance evaluation of protocols for Taenia saginata and Ascaris suum egg recovery from the house fly’s gastrointestinal tract and exoskeleton

Recovery protocola

Sample size (n)

Spiking range

Eggs spiked, average

Flies with 100% recovery

Flies with 0% recovery

Recovery rate (95% CI)b

Time to recovery

Debris scorec

Waiting time

Hands-on time

Phase I: maceration

 G.I.1

15

3–9

5.1

3

0

0.684a (0.580–0.773)

5’

1′30”

2

 G.I.2

14

3–9

5.3

2

1

0.635a (0.478–0.768)

5’

1′30”

2

 G.I.3

15

3–9

5.1

0

3

0.390b (0.277–0.515)

65’

30”

3

Phase II: filtration and flotation

 G.II.1

15

4–8

5.7

6

0

0.849a (0.730–0.921)

5’

1′30”

2

 G.II.2

15

3–8

5.2

4

0

0.795a (0.708–0.861)

15’

4′00”

1

 G.II.3

15

3–9

5.8

0

0

0.425b (0.327–0.530)

15’

6′00”

1

 G.II.4

15

3–9

5.9

0

5

0.193c (0.121–0.293)

60’

1′30”

3

Phase III: centrifugation (part 1)

 G.III.1

15

4–9

6.1

5

0

0.826a (0.705–0.904)

8’

1′30”

2

 G.III.2

14

4–9

6.3

1

0

0.705a (0.600–0.791)

5’

1′30”

2

 G.III.3

15

3–9

5.4

4

0

0.778a (0.666–0.860)

2’

1′30”

2

 G.III.4

15

4–9

6.3

3

1

0.768a (0.653–0.854)

5’

1′30”

2

Phase IV: centrifugation (part 2)

 G.IV.1

20

3–8

5.1

7

0

0.733a (0.622–0.820)

8’

1′30”

2

 G.IV.2

20

3–8

5.2

7

0

0.816a (0.720–0.884)

2’

1′30”

2

  1. ′ Minutes, ″ seconds, CI confidence interval
  2. aG, Gastrointestinal tract; I–IV, phase during which the protocol was evaluated; 1–4, protocol evaluated within that phase
  3. bRecovery rates (ranging from 0 to 1) followed by different lowercase letters within each phase are significantly different from each other at P ≤ 0.05
  4. cA debris score of 1 indicates minimal debris; 2, indicates some debris which necessitates longer reading time but still allows differentiating eggs from debris; 3, indicates a lot of debris which prevents differentiating eggs from debris