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Fig. 5 | Parasites & Vectors

Fig. 5

From: Cross-species immunoprotective antigens (subolesin, ferritin 2 and P0) provide protection against Rhipicephalus sanguineus sensu lato

Fig. 5

Sera cross-recognitions to native Rhipicephalus sanguineus sensu lato SUB, FER2 and P0. By western blot, R. sanguineus sensu lato tissue extracts were analyzed using rabbit sera (1:100) from the control group (a), anti-subolesin group (b), anti-ferritin 2 group (c) and anti-cocktail group (d). Alkaline phosphatase reactions were performed with nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). Anti-IgG alkaline phosphatase rabbit sera conjugate (1:5000) was used as secondary anti-body. E, Egg; L, larvae; MW, molecular weight; N, nymph; nFER2, native ferritin 2; nP0, native P0; nSUB, native subolesin; Ov, ovary; SG, salivary glands; rFER2, recombinant ferritin; rSUB, recombinant subolesin.

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Parasites & Vectors

ISSN: 1756-3305

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