Table 1 Primers for tick-borne pathogens screening, and amplification of tick open reading frame coding genes
Organism | Primer sequence | Amplicon (bp) | References |
|---|---|---|---|
Piroplasms (Theileria/Babesia spp.)/18S rRNA gene | F: ACCGTGCTAATTGTAGGGCTAATAC R: GAACCCAAAGACTTTGATTTCTCTC | 897 | [33] |
Rickettsial gltA | F: GCAAGTATCGGTGAGGATGTAAT R:CTTCCTTAAAATTCAATAAATCAGGAT | 401 | [34] |
Anaplasma spp./16S rRNA gene | F: GGTACCYACAGAAGAAGTCC R: TGCACTCATCGTTTACAG | 345 | [35] |
Tick’s ORF coding genes | |||
Subolesin | F: ATGGCTTGYGCRACATTAAAGCG R: TTACGACAAATAGCTGGGCG | 486 | This study |
P0 | F1: ATGGTCAGGGAGGATAAGAC F2: ATTGTGAACGGCCTGAAAAACCTGA R: YYTAGTCGAAGAGTCCGAAGCCCAT | 957 | This study |
234 | This study | ||
Ferritin 2 | F: ATGGGCAACAACCTGAACGAACAG R: TTAGGTACGCAGCTGCTGATCCAG | 531 | This study |
Actin | F: TCAGGTCATCACCATCGGCAAC R: GTACATGGTGGTGCCGCCG | 184 | [36] |