Groups | Nymphs | | |
|---|
Engorgeda | Weightb | Moltingc | | |
|---|
Control | 31 ± 3.4 | 3.75 ± 0.13 | 26 ± 3 | | |
rSUB | 19.3 ± 2.08 (37.6%*) | 2.92 ± 0.05 (22%*) | 12.33 ± 1.52 (52.5%*) | | |
rFER2 | 16 ± 2.6 (48%*) | 2.93 ± 0.08 (21.7%*) | 9.33 ± 3.75 (64%*) | | |
Cocktail | 22.3 ± 2.08 (27%*) | 3.11 ± 0.19 (17%*) | 12.66 ± 1.54 (51%*) | | |
| | Adult females |
|---|
Engorgeda | Weightb | Egg layingd | Egg fertilitye | Total vaccine efficacyf |
|---|
Control | 5.33 ± 1.15 | 162.9 ± 13.1 | 0.56 ± 0.004 | 0.21 ± 0.035 | |
rSUB | 3 ± 0 (43.75%) | 127.8 ± 5.17 (21.5%*) | 0.26 ± 0.05 (53%*) | 0.138 ± 0.089 (34.3%) | 86.3%g |
rFER2 | 3 ± 0 (43.75%) | 124.1 ± 12.5 (23.8%*) | 0.25 ± 0.03 (55%*) | 0.104 ± 0.031 (50.4%*) | 95.9%h |
Cocktail | 3.33 ± 0.5 (37.5%) | 107.8 ± 23.3 (33.8%*) | 0.32 ± 0.12 (43%*) | 0.095 ± 0.018 (54.8%*) | 90.9%h |
- Values in table are presented as the mean ± standard deviation, with the reduction in percentage given in parentheses
- rFER2 Recombinant ferritin2; rPO recombinant partial 60S acidic ribosomal protein, rSUB recombinant subolesin
- *Significant difference from control at P-value < 0.05 was performed using Student's t-test
- aMean values for engorged nymphs and engorged female ticks fed on rabbits
- bMean values for weight (mg) of engorged nymphs and engorged female ticks
- cMolting capability
- dWhole egg weight per total female’s weight
- eWhole larvae weight per total egg weight
- fVaccine efficacy = 100 × [1 − (RN × VN × RA × OA × FE)], where RN, VN, RA, OA, and FE are mean values of the number of nymphs, molted nymphs, number of engorged females, egg weight and hatched larvae in the immunized group relative to that in the control group, respectively
- gSince the RA and FE were not significantly different in this group, these values were considered to be 0% of protection in the total vaccine efficacy formula
- hSince the RA was not significantly different in this group, this value was considered to be 0% of protection in the total vaccine efficacy formula
