Fig. 7

Growth rate of culturable bacteria in gut of housefly larvae. To determine the growth rate of culturable bacteria in the gut of housefly larvae, bacterial growth tests were carried out in NA medium plates under aerobic culture conditions. BB (EhX, KX,Ec, Lf, Bf and Ab), HB ( Y12, Ef, Sm, PsX and Pv) and NB (Ll) were inoculated in LB liquid medium and cultured at 37 °C overnight (OD₆₀₀ > 1.0). Two 6 mm-diameter sterile filter papers were placed symmetrically on both sides of the agar medium, and 10 µl EhX was added to the filter papers. All plates were cultured at 37 °C under the aerobic condition for 24 (A), 48 (B), 72 (C), 96 (D) and 120 h (E), respectively. The growth diameter of bacteria was recorded. Other bacteria were cultured and the growth diameter measured similarly. The growth rate was evaluated by measuring the colony size. The experiments were conducted with three independent biological replications. The data were compared by one-way analysis of variance. BB, Beneficial bacteria cocktail; BBP, beneficial bacteria phage cocktail; HB, harmful bacteria cocktail; HBP, harmful bacteria phage cocktail; LB, Luria-Bertani (negative control); NA, neutral agar; NB, neutral bacteria