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Fig. 4 | Parasites & Vectors

Fig. 4

From: The diverse functions of Mu-class Glutathione S-transferase HrGSTm1 during the development of Hyalomma rufipes with a focus on the detoxification metabolism of cyhalothrin

Fig. 4

SDS-PAGE electrophoresis of purified proteins from rHrGSTm1. a SDS-PAGE of rHrGSTm1 in Escherichia coli BL21 under the optimized conditions. Lanes: M, Marker; C, bacteria without IPTG addition, swerving as the negative control; 1, 3, 5, 7, production of rHrGSTm1 in the supernatant with IPTG at 37 °C for 2, 4, 6 and 8 h, respectively; 2, 4, 6, 8, production of the rHrGSTm1 in the precipitation with IPTG at 37 °C for 2, 4, 6, 8, respectively. b SDS-PAGE of rHrGSTm1 eluted with different concentrations of imidazole. Lanes: M, Marker; 1, before affinity; 2, after affinity; 3–8, recombinant proteins eluted by 20, 50, 100, 200, 250 and 500 imidazole, respectively. SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; rHrGSTm1, recombinant HrGSTml protein

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Parasites & Vectors

ISSN: 1756-3305

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