Genomic surveillance of Anopheles mosquitoes on the Bijagós Archipelago using custom targeted amplicon sequencing identifies mutations associated with insecticide resistance

Table 3 Position and frequency of detected non-synonymous single-nucleotide polymorphisms in mosquitoes sampled from the Bijagós Archipelago

Chromosome	Amplicon	Position	N^a	Mutation	Genotype frequencies (%)			Alternate allele frequency (%)
Chromosome	Amplicon	Position	N^a	Mutation	Homozygous (reference)	Heterozygous	Homozygous alternate	Alternate allele frequency (%)
2L	vgsc-I	2391191	159	F389L	98.7	1.3	0.0	0.6
	vgsc-I	2391309	160	E429K	58.1	41.9	0.0	20.9
	vgsc-II	2422652	135	L995F^b*	82.2	11.1	6.7	12.2
	vgsc-III	2429745	170	N1570Y^b	88.8	10.0	1.2	6.2
	vgsc-IV	2430424	139	A1746S^b	98.6	1.4	0.0	0.7
2L	rdl	25429236	89	A296G^b	97.8	2.2	0.0	1.1
2R	ace1	3491844	175	P203Q	98.9	1.1	0.0	0.6
		3492205	175	E323D	98.3	1.1	0.6	1.1
3R	gste2	28597858	182	I187F	98.9	1.1	0.0	0.5
		28597896	182	P174L	48.4	2.7	48.9	50.3
		28597905	182	G171D	89.6	9.3	1.1	5.8
		28597956	189	T154S	69.3	21.7	9.0	19.8

^aN is the total number of alleles called for each position
^bThis mutation was one of the 17 targeted insecticide resistance single-nucleotide polymorphisms

ISSN: 1756-3305