Fig. 1

Development of avian embryonic intestinal tissue visualized by hematoxylin and eosin (H&E) histochemistry and immunostaining for marker LGR5. A Avian embryos of several ages were obtained, and intestinal tissues were isolated to observe their morphology. B The development of the small intestine at E9, E11, E13, E15, and E18 were observed by H&E staining. C LGR5 expression by immunofluorescence (IF) detection. The nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI, blue), and LGR5 was stained with anti-LGR5 (green). Scale bars: 100 μm