Fig. 2

Morphology of primary epithelial cells isolated from E9. A Cellular morphology in 12, 48, and 120 h of culture after isolation. B SV40T antigen was successfully transfected into embryonic kidney epithelial cells (293FT) and integrated into the small avian intestinal epithelial cell line (AIEC) of E9 embryos. C F10 and F30 AIEC marker genes (KRT-18, CDH1, CLDN1, TJP1, OCLN, and VILL) expression detection. D Morphology of F5, F10, F20, and F30 AIEC (light/green). E Representative images of immunofluorescence staining of F30 AIEC for TJP1 showing a single cell layer, including nuclei of cells stained in blue (HoChest33342) and TJP1 in red. The images were captured with a fluorescence microscope. Scale bars: 50 μm