Table 1 Summary of target gene primers used for PCR and qPCR analysis
Gene | Primer sequence (5′ to 3′) | Product size (base pairs) | Accession number |
|---|---|---|---|
CCNE2 | F: ACCAGGAAAAGAAGAACGGCA | 129 | NM_001030945.2 |
| Â | R: GATGATGCAAGGCGAGATGC | Â | Â |
CDC25A | F: CTCGCCGGTCTCAGATCTTC | 247 | XM_001199572.1 |
| Â | R: TCTTCTGAGAGGCAGTCGGA | Â | Â |
CDH1 | F: AGCCAAGGGCCTGGATTATG | 230 | NM_001039258.3 |
| Â | R: GGTGAATGTACAGCCAGCCT | Â | Â |
CDK1 | F: CGAGCCTTTGGAATCCCAGT | 249 | XM_015288063.3 |
| Â | R: GGATTCCACATCAGGCCACA | Â | Â |
CDKN1A | F: AGCAGCTGGAAGAAGCTCAG | 289 | NM_204396.2 |
| Â | R: TCTTTGATGGTGGTCTGCCC | Â | Â |
CLDN1 | F: ACCCACAGCCTAAGTGCTTC | 200 | NM_001013611.2 |
| Â | R: AGGTCTCATAAGGCCCCACT | Â | Â |
GTSE1 | F: GAGGGAGACGTTCTGTGTGG | 182 | NM_001031332.2 |
| Â | R: GTTCCTGTGTCAGAGGCGAA | Â | Â |
KRT-18 | F: GCCAAGAAGAACGTGGAGGA | 290 | XM_025145666.1 |
| Â | R: GCGCAGAGCATCCAGGTC | Â | Â |
OCLN | F: GTGTAAGGCCCACACCTCTG | 187 | NM_205128 |
| Â | R: ATGCCTTCCCAAAAAGCCCT | Â | Â |
TJP1 | F: ACTGTGACCCCAAAACCTGG | 294 | XM_040680632.1 |
| Â | R: CTCCCTGCTTGTGGCATGTA | Â | Â |
TP53I3 | F: CTTGAGAACTGATGGCCGGT | 217 | XM_040698015.1 |
| Â | R: AAATGGCTGGAGATGTGGGG | Â | Â |
VILL | F: CGTGCACGTTACCTAACACAG | 181 | XM_418521 |
| Â | R: TTACCCACACCCAGTCATGC | Â | Â |
GallusGAPDH | F: GGTGGCCATCAATGATCCCT | 105 | NM_204305.2 |
| Â | R: CCGTTCTCAGCCTTGACAGT | Â | Â |
EtGAPDH | F: TGGAGTCTTCACGAACAAGGA | 109 | XM_013378585.1 |
| Â | R: ACCCATCACAAACATCGGAGTA | Â | Â |