Fig. 1

A 20-week HSHF dietary intervention induced metabolic syndrome symptoms in C57 mice. Mice were fed with either a CHOW or an HSHF diet for 20 weeks. Over the course of the experiment, A weight gain was monitored weekly, while B insulin and C glucose tolerance were evaluated in the 19th and 20th weeks, respectively. By the end of the dietary intervention, blood was collected, and D insulin, E glucose, F triglycerides, G total cholesterol, and H AST/ALT levels were measured. At least six mice were used in each experiment (A–H). Unpaired t-test was performed. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001