Fig. 2

Mosquitoes fed on an HFHS mouse had a reduced survival rate. Mice were fed with either a CHOW or an HSHF diet for 20 weeks. Over the course of the experiment, metabolic syndrome was followed by tracking weight gain, glucose, and insulin sensitivity. Then, Aedes mosquitoes were allowed to feed on anesthetized mice, and A daily survival was measured over the course of 30 days. Alternatively, whole-body relative expression levels of B TOR, C insulin receptor, and D FoxO was measured by qRT-PCR 1 and 4 days pbm. Four biological replicates were prepared using independent mosquito hatches and dietary protocols. Sugar-fed (SF) mosquitoes were used as a baseline for survival and gene expression. A Mantel-Cox test and B–D one-way ANOVA followed by Tukey’s multiple comparison tests were performed. ns: non-significant, *P < 0.05, ***P < 0.001