Fig. 3

Mosquitoes fed on an HFHS mouse had an increased oviposition and lipid uptake. Mice were fed with either a CHOW or an HSHF diet for 20 weeks. Over the course of the experiment, metabolic syndrome was followed by tracking weight gain, glucose, and insulin sensitivity. Aedes aegypti mosquitoes were allowed to feed on anesthetized mice, and A egg layering was measured at 5 days pbm. Following a blood meal, mosquitoes were dissected, and TAG content was measured in the B fat body and C ovaries at 2 and 3 days pbm, respectively. Whole-body relative expression levels of D lipophorin and E vitellogenin were measured by qRT-PCR 1 and 4 days pbm. Sugar-fed (SF) mosquitoes were used as a baseline for gene expression. Four biological replicates were prepared using independent mosquito hatches and dietary protocols. A–C Unpaired t-test was performed and D–E one-way ANOVA followed by Tukey’s multiple comparison tests were performed. ns: non-significant, *P < 0.05, **P < 0.01, ***P < 0.001