Table 1 Sequences, annealing temperatures, and product size of PCR primers used in the current studya
From: Strongyloides stercoralis genotyping in a human population in southwestern Iran
Target gene | Primers | Nucleotide sequences (5′–3′) | Annealing temp (°C) | Product length |
|---|---|---|---|---|
18S rRNA (HVR-I) | Forward (RH5401) | AAACATGAAACCGCGGAAAG | 52 | 862 bp |
Reverse (RH5402) | CATTCTTGGCAAATGCTTTCG | |||
Sequencing (RH5403) | AGCTGGAATTACCGCGGCTG | |||
18S rRNA (HVR-IV) | Forward (18SP4F) | GCGAAAGCATTTGCCAA | 57 | 712 bp |
Reverse (18SPCR) | ACGGCCGGTGTGTAC | |||
Sequencing (ZS6269) | GTGGTGCATGGCCGTTC | |||
cox-1 | Forward (ZS6985) | GGTGGTTTTGGTAATTGAATG | 47 | 837 bp |
Reverse (ZS6986) | ACCAGTYAAACCACCAATAGTAA | |||
Sequencing (ZS6990) | GGTTGATAAACTATAACAGTACC |