Fig. 1

Visualization of fluorescent trypanosomes in tsetse fly organs. Red and green fluorescent Trypanosoma congolense savannah (TCS) in proventriculus (A) and midgut (B) from a tsetse fly dissected 25 days post-infection with a mixture of Gam2 RFP and 1/148 GFP. C Autofluorescence of the tsetse proboscis; a portion of an uninfected labrum is shown. Rows top to bottom: phase contrast, green fluorescence, red fluorescence, merge of red and green fluorescence, merge all. Areas of overlapping red and green fluorescence appear yellow in the merged images of the proventriculus (A) and midgut (B), but no yellow fluorescent trypanosomes were seen when the preparations were squashed under the coverslip to separate individual trypanosomes. Scale bar = 50 µm