Fig. 3

Morphology of yellow fluorescent TCS epimastigotes and trypomastigotes from proboscides. Examples of yellow fluorescent trypanosomes from proboscides of tsetse infected with Gam2 RFP and 1/148 GFP (dissected 28 days post-infection) and stained live with Hoechst 33342. Epimastigotes (A–F) varied greatly in morphology; some cells were replicating as they had two kinetoplasts and one or two nuclei (E, F). Trypomastigotes (G–I) were usually long and often had pronounced undulations at the anterior end. From left to right: phase contrast, Hoechst 33342, merge, green fluorescence, red fluorescence, merge of red and green fluorescence. Arrows indicate kinetoplasts. Scale bar = 10 µm