Fig. 8

In vitro mixing of red and green fluorescent epimastigotes; 1/148 RFP and Gam2 GFP epimastigotes were mixed in vitro, but very few groups of red and green trypanosomes such as that shown in A were found, most clumps being a single colour. Co-cultures were found to contain a few yellow fluorescent trypanosomes (arrowed) at 21 days post-mixing (B–D), presumably resulting from cytoplasmic exchange between cells of the different strains. From left to right: phase contrast, green fluorescence, red fluorescence, merge of red and green fluorescence. A Scale bar = 20 µm; B–D scale bar = 10 µm