Experimental genetic crosses in tsetse flies of the livestock pathogen Trypanosoma congolense savannah

Parasites & Vectors

Table 2 Summary of five successful interclonal Trypanosoma congolense savannah crosses and one intraclonal cross

Parent 1	Parent 2	No. infected proboscides	Fluorescence of trypanosomes in proboscis
Parent 1	Parent 2	No. infected proboscides	NF	R	G	RG	RGY
WG81 RFP	1/148 GFP	23	0	12 (52%)	0	10 (44%)^a	1 (4%)
Gam2 RFP	WG81 GFP	19	4 (21%)	1 (5%)^a	10 (53%)^b	1 (5%)	3 (16%)^a,c
Gam2 RFP	1/148 GFP	33	1 (3%)	0	11 (33%)^a	11 (33%)	10 (31%)^a,b
1/148 RFP	WG81 GFP	31	7 (23%)	3 (10%)^a	10 (32%)^a	1 (3%)	10 (32%)^a,b
1/148 RFP	Gam2 GFP	23	5 (22%)	16 (70%)^a	0	1 (4%)	1 (4%)
1/148 RFP	1/148 GFP	11	2 (18%)	3 (27%)^a	0	5 (46%)	1 (9%)

NF: non-fluorescent trypanosomes; R: red fluorescent; G: green fluorescent; RG: both red and green fluorescent; RGY: red, green and yellow fluorescent
^aNon-fluorescent trypanosomes also present
^bNo red fluorescent trypanosomes were seen in two flies
^cNo red fluorescent trypanosomes were seen in one fly

ISSN: 1756-3305