Fig. 2

Primer selection for MIRA of T. vaginalis repetitive DNA sequence. A Screening of the optimal reverse primer using TV-F3 as the forward primer. B Screening of the optimal forward primer using TV-R5 as the reverse primer. C Sensitivity testing of the TV-F5/TV-R5 primer pair using positive plasmid template concentrations as follows. 1: 1 × 10^–2 ng/μl;2: 1 × 10^–3 ng/μl; 3: 1 × 10^–4 ng/μl; 4: 1 × 10^–5 ng/μl; 5: 1 × 10^–6 ng/μl; 6: 1 × 10^–7 ng/μl. NC negative control