Fig. 3From: A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalisMIRA time and temperature optimization. A Optimal reaction time for MIRA assay. Saturation amplification was achieved within 30 min. B Temperature optimization for MIRA from 25 °C to 45 °C; the highest amplification efficiency was observed at 37 °C. NC negative controlBack to article page