Fig. 4
MIRA-CRISPR/Cas13a-LFD system test for T. vaginalis detection. A MIRA-CRISPR/Cas13a-LFD system test, NC: negative control using ddH2O as template. Plasmid: testing group using 1 × 10–6 ng/μl positive plasmid as the template. B, C MIRA-CRISPR/Cas13a-LFD assay FAM-UUUUUUUUUUUUU-biotin reporter (13U-FB-reporter) and FAM-UUUUUU-biotin reporter (6U-FB-reporter) test. 1. Negative control using ddH2O as template. 2. 1 × 10–6 ng/μl positive plasmid as the template. 3. 1 × 10–3 ng/μl positive plasmid as the template