Table 1 Primers and probes used in this study

TV-F1

TCCATTAAGAAACCTCAAGAGATGACAAGAG

TV-F2

ATTGCTTATGAATTCGACATGGGATATTCC

TV-F3

ACCCCAAACTTGCAATGACACTCAAATTGC

TV-R1

TAATACGACTCACTATAGGGACTGAGGGTAAGACCAATGTTCGACAATAG

TV-R2

TAATACGACTCACTATAGGGTTGTTGTAGTTGTCAAGGACTGCCTTTGCG

Candidates for MIRA primers screened in this study

TV-F5/TV-R5 pair was used for follow-up experimental tests

(this paper)

TV-R3

TAATACGACTCACTATAGGGACCTTCACTTTGGATTTGACTTCGGAGAAG

TV-F4

TCGACATGGGATATTCCATTAAGAAACCTC

TV-F5

ACTTGCAATGACACTCAAATTGCTTATGAA

TV-F6

ACACCCCAAACTTGCAATGACACTCAAATTGCTTA

TV-F7

TGCAATGACACTCAAATTGCTTATGAATTCG

TV-R4

TAATACGACTCACTATAGGGTTGCGAACTGAGGGTAAGACCAATGTTCGACAATAGGT

TV-R5

TAATACGACTCACTATAGGGTACTAAGCCACTTTGACCTTGAGTGAATTCGTACTTC

TV-R6

TAATACGACTCACTATAGGGAATGTTCGACAATAGGTTTTAATGTGGTTACTAAGCCAC

TV-R7

TAATACGACTCACTATAGGGAGACCAATGTTCGACAATAG

TV-crRNA

GAUUUAGACUACCCCAAAAACGAAGGGGACUAAAACUUAUGAGAAGGUCGACAACUUUGUUGAA

crRNA

(this paper)

13U-FB-reporter

FAM-UUUUUUUUUUUUU-Bio

Reporter

6U-FB-reporter

FAM-UUUUUU-Bio

TV-8S

TCTGGAATGGCTGAAGAAGACG

Nested PCR primers [26] (actin gene)

TV-9R

CAGGGTACATCGTATTGGTC

TV-10S

CAGACACTCGTTATCG

TV-11R

CGGTGAACGATGGATG