Table 1 Primers used in different PCR of the Vgsc gene of Aedes aegypti from Iran
Primer | Purpose | Sequence (5′ to 3′) | Length | Refs. |
|---|---|---|---|---|
V410fw | Allele-specific PCR, melting curve analysis, wild genotype (IS6) | GCGGGCAGGGCGGCGGGGGCGGGGCCA TCTTCTTGGGTTCGTTCTACCGTG | 51 | [55] |
L410fw | Allele-specific PCR, melting curve analysis, mutant genotype (IS6) | GCGGGCATCTTCTTGGGTTCGTTCTACCATT | 31 | [55] |
410rev | Allele-specific PCR, melting curve analysis, reverse primer (IS6) | TTCTTCCTCGGCGGCCTCTT | 20 | [55] |
410fw | Sequencing V410L position | GATAATCCAAATTACGGGTATAC | 23 | [55] |
kdrseq-F | PCR and sequencing (IIS6) | AGACAATGTGGATCGCTTC | 19 | Investigator-designed |
kdrseq-R | PCR and sequencing (IIS6) | ACGCAATCTGGCTTGTTA | 18 | Investigator-designed |
kdrext-F | Allele-specific PCR (IIIS6) | CGAACTTGTTACGAATGATCTGCTTAC | 27 | [64] |
kdrext-R | Allele-specific PCR (IIIS6) | TGAGAATAGCGAGATTAGGAAGGAA | 25 | [64] |
wildint-F | Allele-specific PCR (IIIS6) | CCTCTACTTTGTGTTCTTCATCATTTG | 27 | [64] |
mutint-R | Allele-specific PCR (IIIS6) | GCGTGAAGAACGACCTGA | 18 | [64] |