Fig. 2

Chronogram with age estimates of major divergence events among Amblyomma spp., based on the mitogenomic data set using Bayesian relaxed dating methods (BEAST) and topology 2 (Additional file 1: Fig. S1b). Horizontal bars represent 95% credibility intervals of relevant nodes, and the four calibration constraints are indicated in red with a number and asterisk on the corresponding nodes following the corresponding order date of the text. Dates (and credibility intervals) are in millions of years. Origin and diversification of Amblyomma are highlighted with a vertical broken line. Periods on the geological table are shown in different colors. The specimens sequenced in this study are highlighted in bold font. Species with the same subgeneric status are indicated in the same color. IAA, lineage with Indomalayan, Afrotropical, and Australasian distributions; My, million years; Ple, Pleistocene; Pli, Plicene; Q, Quaternary