Fig. 4

A Representation of the results of finished reaction tubes (Ov-RPA–CRISPR/Cas12a) when exposed to UV light. On the basis of KK and/or FECT methods, tube NC contained O. viverrini-negative human copro-DNA, and tubes 1, 2, 5, 6, 7, 8, 10, 13, and 14 were positive for O. viverrini-like eggs. Tube 4 showed positive results for the coinfection of O. viverrini-like eggs and hookworms. Tubes 9 and 12 exhibited positivity for the coinfection of O. viverrini-like eggs and Taenia sp., respectively. Tubes 3 and 11 were positive for hookworms and S. stercoralis, respectively. Tube 15 represented a negative fecal sample, whereas tube PC served as a positive control with a plasmid containing the O. viverrini NAD1 gene. All reaction tubes were visually inspected using an UV transilluminator and photographed using a phone camera. B The image was recorded using the Gel Doc XR + Gel Documentation System. The tube’s label is based on image A. C The image shows agarose gel electrophoresis results of RPA products, with the red arrow indicating the target amplicon (281 bp). The lane labels align with images A and B. Additional details, including the results of KK and/or FECT methods, number of eggs per gram (NEPG), and Ov-RPA–CRISPR/Cas12a assay, are shown below the image. Lane M: 1 kb ladder. 1% gel electrophoresis (0.5× TBE, 100 V, ~ 35 min)