Fig. 7

Depletion of PPKL resulted in defects in parasite replication and morphology in the type II strain ME49. A, B Parasites were grown for 24 h in the absence or presence of auxin, followed by IFA using antibodies against MLC1, IMC1, actin, tubulin, and acetylated tubulin. The antibodies against MLC1 and IMC1 served as the controls for the IFA. Scale bar = 2 μm. C, D Parasites with depleted PPKL were not associated with differentiation of ME49. Parasites were grown on HFF in 1% FBS in pH8.1 μm