Fig. 4

Unsupervised clustering of proteins identified in the Rhipicephalus microplus saliva proteome throughout blood feeding. A The patterns were identified through clustering analysis using the CLICK algorithm. The x-axis represents the tick groups, the y-axis represents log2-based ratios standardized expression levels of cluster groups, and error bars represent the standard error. Each cluster is presented with triplicate samples. In parentheses, the number of proteins identified in each cluster is shown. B The frequency of the functional classes of proteins identified within each cluster is represented by the size of each sphere