A molecular assessment of Ostertagia leptospicularis and Spiculopteragia asymmetrica among wild fallow deer in Northern Ireland and implications for false detection of livestock-associated species

Table 2 False-positive PCR hits from species-specific primer sets

Host	Target species	Primers tested*	Ostertagia leptospicularis hits?**	Spiculopteragia asymmetrica hits?**
Cattle	Cooperia onchophora	2
Cattle	Ostertagia ostertagi	4	1
Sheep	Chabertia ovina	2
Sheep	Cooperia curticei	3
Sheep	Haemonchus contortus	5	1	1
Sheep	Oesophagostomum venulosum	3
Sheep	Teladorsagia circumcincta	5	1	1
Sheep	Trichostrongylus axei	3	1	1
Sheep	Trichostrongylus colubriformis	3	1	1
Sheep	Trichostrongylus vitrinus	2	2	2
na	Pan-nematode (+ control)	2	2	2

*Primers shown in Additional file 2: Table S1 and representative result gel images shown in Fig. 2. **Single PCR products indistinguishable in size compared to positive controls

ISSN: 1756-3305