Table 2 Comparison of free amino acids between sparganum-infected and noninfected muscle of Rana limnocharis (mg/ml, N = 3)

1

Phosphoserine (P-Ser)

5.08 ± 0.36

5.75 ± 1.03

n.s. (0.343)

2

Taurine (Tau)

15.33 ± 7.23

17.50 ± 7.31

n.s. (0.733)

3

Phosphorylethanolamine (PEA)

0.95 ± 0.24

1.55 ± 0.11

< 0.05 (0.017)

4

Urea (Ure)

374.06 ± 83.94

341.80 ± 73.31

n.s. (0.798)

5

Aspartic acid (Asp)^a

5.87 ± 1.44

8.79 ± 1.16

n.s. (0.053)

6

Threonine (Thr)^a

15.82 ± 1.81

25.14 ± 2.94

< 0.05 (0.009)

7

Serine (Ser)^a

19.78 ± 2.41

30.86 ± 2.14

< 0.05 (0.004)

8

Asparagine (Asn)^a

12.78 ± 0.67

20.76 ± 1.78

< 0.05 (0.012)

9

Glutamic acid (Glu)^a

18.18 ± 2.38

33.20 ± 3.74

< 0.05 (0.004)

10

Glutamine (Gln)^a

28.79 ± 2.62

52.65 ± 6.48

< 0.05 (0.004)

11

Amino ethylenediamine (α-AAA)

1.00 ± 0.42

3.06 ± 1.04

< 0.05 (0.033)

12

Glycine (Gly)^a

19.06 ± 2.41

23.70 ± 5.46

n.s. (0.249)

13

Alanine (Ala)^a

55.33 ± 1.72

74.50 ± 6.82

< 0.05 (0.009)

14

Aminobutyric acid (α-ABA)

0.56 ± 0.37

1.25 ± 0.62

n.s. (0.172)

15

Valine (Val)^a

21.44 ± 2.13

31.07 ± 4.10

< 0.05 (0.022)

16

Cystine (Cys)^a

1.36 ± 0.55

3.84 ± 1.33

< 0.05 (0.04)

17

Methionine (Met)^a

12.84 ± 0.96

21.86 ± 6.56

n.s. (0.078)

18

Isoleucine (Ile)^a

15.13 ± 1.18

22.25 ± 2.83

< 0.05(0.016)

19

Leucine (Leu)^c

43.15 ± 4.43

73.12 ± 21.07

n.s. (0.073)

20

Tyrosine (Tyr)^b

21.14 ± 3.02

34.31 ± 7.39

< 0.05 (0.046)

21

Phenylalanine (Phe)^b

17.63 ± 3.52

30.26 ± 6.50

< 0.05 (0.042)

22

Alanine (β-Ala)

10.27 ± 0.69

16.17 ± 6.35

n.s. (0.185)

23

Aminoisobutyric acid (β-AiBA)

1.00 ± 0.49

6.88 ± 5.47

n.s. (0.137)

24

Aminobutyric acid (γ-ABA)

2.75 ± 0.50

5.84 ± 3.26

n.s. (0.181)

25

Histidine (His)^a

15.29 ± 1.62

21.48 ± 2.44

< 0.05 (0.022)

26

Carnosine (Car)

924.93 ± 50.36

776.61 ± 116.52

n.s. (0.113)

27

Tryptophan (Trp)^b

3.85 ± 2.81

1.54 ± 0.88

n.s. (0.360)

28

Ornithine (Orn)

4.93 ± 3.21

6.38 ± 2.22

n.s. (0.555)

29

Lysine (Lys)^c

62.33 ± 18.10

80.85 ± 7.66

n.s. (0.160)

30

Arginine (Arg)^a

37.40 ± 10.75

50.52 ± 10.58

n.s. (0.207)

31

Proline (Pro)^a

7.83 ± 1.89

16.92 ± 1.44

< 0.05 (0.003)

32

Total amino acid

1775.62 ± 237.25

1837.16 ± 292.69

n.s. (0.791)