Fig. 4
General carbohydrate measures. A Anthrone colorimetric reactions for demonstrating color saturation changes with respect to metabolized sucrose. B Carbohydrate estimates in adult mosquitoes fed water (H2O), 5% sucrose (5%S), 10% sucrose (10%S), 5% erythritol (5%E), 10% erythritol (10%E), and 5% erythritol + 5% sucrose (5%E + 5%S). Samples were homogenized in phosphate-buffered saline followed by centrifugation, and the supernatant was used on reaction plates in a spectrophotometer. Values per mosquito were estimated using the standard curve sucrose controls run on the same reaction plate. Bar graphs are represented with I-bars as standard error of the mean. Kruskal–Wallis and Wilcoxon signed-rank test (H5,26 = 14.1373, P < 0.0148) annotated with an asterisk