Table 2 Primer sequences and concentrations
Primer | Primer sequence (5’–3’) | Total provision (O.D.) | Optimal concentrations (μM) | Length |
|---|---|---|---|---|
RLB-F | AGYGGCGGACGGGTGAGTAA | 5 | 50 | 1100 bp |
RLB-R | Biotin-CCATTGTAGCACGTGTGTAGCCC | 5 | 50 |
Primer | Primer sequence (5’–3’) | Total provision (O.D.) | Optimal concentrations (μM) | Length |
|---|---|---|---|---|
RLB-F | AGYGGCGGACGGGTGAGTAA | 5 | 50 | 1100 bp |
RLB-R | Biotin-CCATTGTAGCACGTGTGTAGCCC | 5 | 50 |