Table 3 Probe sequences and concentrations
Probe | Probe sequence (5’–3’) | Accession no. | Base number | Total provision (O.D.) | Optimal concentrations (μM) | Position |
|---|---|---|---|---|---|---|
Catch-all | (NH2)-CAGGATTAGATACCCTGGTAGTCC | – | 24 | 10 | 50 | 820–843 bp |
Staphylococcus aureus-1 | (NH2)-TCAAAAGTGAAAGACGGTCTTGC | NR118997 | 23 | 10 | – | 220–242 bp |
Staphylococcus aureus-2 | (NH2)-CAACATATGTGTAAGTAACTGTGCAC | NR118997 | 26 | 10 | 50 | 480–505 bp |
Shigella flexneri-1 | (NH2)-GGAGTAAAGTTAATACCTTTGC | X96963 | 22 | 10 | – | 480–501 bp |
Shigella flexneri-2 | (NH2)-CTGATACTGGCAAGCTTGAGTCTCGT | X96963 | 26 | 10 | 50 | 670–695 bp |
Aeromonas caviae-1 | (NH2)-CGAGGAGGAAAGGTCAGTAGC | NR029252 | 21 | 10 | – | 108–128 bp |
Aeromonas caviae-2 | (NH2)-GGAATCAGAACACAGGTGCT | NR029252 | 20 | 10 | 100 | 698–717 bp |
Vibrio vulnificus | (NH2)-AGAGAATTCTAGCGGAGACGCG | NR118930 | 22 | 10 | 100 | 665–686 bp |
Salmonella enterica | (NH2)-AGAAGAATCCAGAGATGGATTG | NR119108 | 22 | 10 | 100 | 666–687 bp |
Proteus vulgaris-1 | (NH2)-GGTGATAAAGTTAATACCTTTGTCAA | NR115878 | 26 | 10 | 100 | 118–143 bp |
Proteus vulgaris-2 | (NH2)-CGAATCCTTTAGAGATAGAGGA | NR115878 | 22 | 10 | – | 667–688 bp |
Yersinia enterocolitica-1 | (NH2)-GGCCAATAACTTAATAGGTTG | NR074308 | 21 | 10 | – | 118–138 bp |
Yersinia enterocolitica-2 | (NH2)-AGAACTTAGCAGAGATGCTTCG | NR074308 | 22 | 10 | 100 | 667–688 bp |