Fig. 3

Toxicity assessment of MMV1794206, monepantel and moxidectin on human hepatoma (HepG2) cells, with reference to two positive controls, doxorubicin (Dox; cytotoxic) and M-666 (mitotoxic). For each compound, the (A) half-maximal cytotoxic concentration (CC₅₀) and (B) half-maximal mitotoxic concentration (MC₅₀) were established via a cell viability assay after 48 h of incubation. Crystal violet staining was used to measure the absorbance (595 nm) of treated cells, which was negative- (blank) and baseline- (100% cell viability) corrected. Data points represent triplicates and are presented as a mean ± standard deviation (SD)