Fig. 5
From: Expression of sex-specific molecular markers by Babesia bovis gametes
Live immunofluorescence detection of TRAP2-4 expression in the surface of female-enriched gametes. a Babesia bovis male gamete incubated with anti-HAP2 and b–e anti TRAP1-4 proteins. GFP-TF5480: transfected B. bovis parasites. Alexa Fluor 647: goat anti-rabbit antibody. Hoechst: nucleic acid stain. Negative control preimmune (PI) rabbit serum as primary antibody and stained with Hoechst. Bars, 5 μm