Fig. 3

Ineffectiveness of m-AB169 (1640) in maintaining the oviposition of S. mansoni compared with AB169. A Fast Blue BB staining (red) and DAPI labeling (gray) showing the vitellaria (left) and ovary (right) of adult female S. mansoni cultured in AB169 and m-AB169 (1640) on D12. Representative images from three experiments with n ≥ 15 parasites. B The sizes (area) of female ovaries. n ≥ 15 worms for each group. C Eggs laid by paired adult females cultured in AB169 and m-AB169 (1640) on D12. The arrows indicate the presence of malformed eggs. Representative of three experiments. D Eggs laid per day per paired parasite cultured in AB169 and m-AB169 (1640) on D12. n ≥ 15 females for each group. E Sizes of eggs laid by parasites cultured in AB169 (n = 24) or m-AB169 (1640) (n = 17) on D12. ns, not significant, *P < 0.05, t test. Error bars represent the SD