Fig. 5

m-AB169 (1640) serving as an RNAi platform for functional studies on the reproduction of S. japonicum. A Schematic of male gli1 RNAi experiments. B qPCR showing expression levels of gli1 and gli1-dependent nrps in the gli1 RNAi group and cntl RNAi group. C Fast Blue BB (red) and DAPI labeling (gray) showing the vitellaria (left) and ovary (right) from female parasites on D28 after pairing with gli1 RNAi or control RNAi male worms. n ≥ 3 experiments with n ≥ 23 worms for each group. D The sizes (area) of the female ovaries from gli1 RNAi group and cntl RNAi group. n ≥ 23 females for each group. E Eggs laid per day per female parasite after pairing with the gli1 RNAi or cntl RNAi males. n ≥ 23 females for each group; The total number of eggs produced in 26 days was counted for calculation. F Schematic of female vf1 RNAi in m-AB169 (1640) supplemented with 100 μΜ BATT. G qPCR showing expression levels of vf1 in vf1 RNAi group and control RNAi group. H Fast Blue BB (red) and DAPI labeling (gray) showing the vitellaria (left) and ovary (right) from the vf1 RNAi and cntl RNAi female parasites on D19. n ≥ 3 experiments with n ≥ 16 worms for each group. I The sizes (area) of ovaries from the vf1 RNAi and control RNAi female parasites. n ≥ 16 females for each group. J Eggs laid per day per female parasite of vf1 RNAi and control RNAi female parasites. n ≥ 16 females for each group. The total number of eggs produced in 19 days was counted for calculation. ns not significant, **P < 0.01; ***P < 0.001; ****P < 0.0001, t test; Error bars represent the SD based on three separate experiments