Fig. 4
From: Construction of luciferase-expressing Neospora caninum and drug screening
In vitro anti-N. caninum determination using Nc1-Luc as a tool. A–C Inhibition of N. caninum (Nc1-Luc) after treatment with atovaquone (A), pyrimethamine (B), or penicillin G potassium (C). Nc1-Luc strains were treated with different concentrations of drugs, and RLUs were detected after 72 h. D–F Cell viability of HFF cells upon treatment with atovaquone (D), pyrimethamine (E), or penicillin G potassium (F). HFF cells were treated with different concentrations of drugs, and absorbance was measured at 450 nm after 72 h. Results are shown as mean ± SD from three independent assays. IC50 and CC50 were calculated using the log (inhibitor) versus normalized response-variable slope in GraphPad Prism (San Diego, CA, USA)