Fig. 6
From: Construction of luciferase-expressing Neospora caninum and drug screening
Effects of TAK-632 on the morphology and division of N. caninum. HFFs were invaded using 5 × 105 Nc1 tachyzoites and then treated with DMSO or TAK-632. A IFA observation of IMC division of tachyzoites by. SRS2 was used to label parental cells of Nc1 tachyzoites. IMC1 was used to label both parental and budding daughter cells. Hoechst was used to stain the nuclei. Arrows indicate tachyzoites with abnormal IMC division. Scale bar, 5 μm. B IFA observation of apicoplast division of tachyzoites. ENR was used to label the apicoplasts of Nc1 tachyzoites. Arrows indicate tachyzoites with abnormal apicoplasts. Scale bar, 5 μm. C Proportion of vacuoles with abnormal IMC divisions. The field of view was randomly selected to count the number of vacuoles with abnormal IMC division. Abnormal ratio = (number of abnormal vacuoles/total number of vacuoles) × 100%. D Proportion of vacuoles with abnormal apicoplast division. Results were tested by t-test. ***P < 0.001