Table 1 The primers of different target genes
From: Circulating cell-free DNA as a biomarker for diagnosis of Schistosomiasis japonica
Scafold/genome | Region/genes | Forward primer | Reverse primer | Product size |
|---|---|---|---|---|
SKCS01001135.1 | TD-1 | GGGGCACGAGGTGTATGAAA | GGTATAGTGACGCAGC | 82Â bp |
SKCS01000032.1 | TD-2 | GCACGACATTGCCCCCTATTTTT | TTCCCCATTTAAAGATGATGCGAG | 90Â bp |
SKCS01001415.1 | TD-3 | ACTCATCACCGCCAATCAAC | TGCACAACCTTCTTCCCCATA | 72Â bp |
SKCS01000951.1 | TD-4 | AAGGCCGATAGTTTACGCGA | AGGTGACAGGGTTTGCTTCTT | 114Â bp |
JQ781208.1 | COX3 | GCTATGCGTGCTCCTACTCC | TTCAGACCCCAGAAGCAACC | 70Â bp |
CYTB | TGAGCTGCTACTGTATTGACTTCT | ACACGAATAAGCGTATCAACCA | 116Â bp | |
COX1(1) | CGTGCGGCTGATCCAATACT | CCCAGTAACACCACCAACTGT | 72Â bp | |
ATP6 | ATACCAGTGGGTTGTCCGAT | ACGGACGGACCAATAAAACGA | 97Â bp | |
COX1(2) | ATGTGCTGTCTTTGGGTTCT | TGGCAACCCATGAACACCTA | 176Â bp | |
| Â | ND5 | AAGCTATGCGTGCTCCTACTC | CAGACCCCAGAAGCAACCAA | 70Â bp |
| Â | SjR2 | CCATCGATATCTGCTGCATCTC | CAAGGTGAAGTCAAGCGAATGA | Â |
| Â | ITS2 | TGATGCGCTCGTATATATGAATGC | GGACTAGGCTGTGGTCGGATT | Â |
| Â | CYTB | TGAGCTGCTACTGTATTGACTTCT | ACACGAATAAGCGTATCAACCA | Â |